Sigma R5886 RPMI-1640 Medium
sterility:sterile-filtered.
form:liquid.
technique(s):cell culture | mammalian: suitable.
impurities:endotoxin, tested;pH>7.2.
components:L-glutamine: no;NaHCO3: yes;sodium pyruvate: no;HEPES: 25 mM;phenol red: yes.
General description:Sigma R5886 RPMI-1640 Medium RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.
Application:
RPMI-1640 Medium has been used:
to culture human lymphoblastoid cell line cells
prostate cancer cells
monocytes from human blood


























永兴县|
建昌县|
襄樊市|
峨边|
富顺县|
南城县|
平潭县|
康平县|
进贤县|
雷州市|
盐源县|
芮城县|
峨边|
石渠县|
蕉岭县|
英德市|
喜德县|
西青区|
嘉禾县|
汉寿县|
河曲县|
屯昌县|
扶余县|
大邑县|
深州市|
万年县|
射洪县|
鲁甸县|
太康县|
张家川|
汕尾市|
汤阴县|
江安县|
百色市|
拉孜县|
武强县|
科技|
建昌县|
杂多县|
如东县|
香河县|