功能、操作簡便、文獻眾多的葉綠素熒光成像系統(tǒng)

突變株快速篩選的工具

主要功能

?  成像功能：對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等參數(shù)進行成像分析。測定調(diào)節(jié)性能量耗散Y(NPQ)，反映植物光保護能力，測定非調(diào)節(jié)性能量耗散Y(NO)，反映植物光損傷程度。

?  程序測量功能：可程序測量熒光誘導曲線、快速光曲線和暗弛豫，也可手動測量；在測量過程中能自動分析所有熒光參數(shù)的變化趨勢

?  AOI功能：可在測量前或測量后任意選擇感興趣的區(qū)域（AOI），程序?qū)⒆詣訉x擇的AOI的數(shù)據(jù)進行變化趨勢分析，并在報告文件中顯示相關AOI的數(shù)據(jù)。所有報告文件中顯示的數(shù)據(jù)都可導出到EXCEL文件中。

?  成像異質(zhì)性分析功能：對任意參數(shù)任意時間的成像，可在圖像上任意選取兩點，軟件自動對兩點間的數(shù)據(jù)進行橫向異質(zhì)性分析，并可導出到EXCEL文件中。

?  成像數(shù)據(jù)范圍分析功能：對任意參數(shù)任意時間的成像，可分析任意兩個熒光數(shù)值之間有多少個像素點，多少面積（cm2）。

?  突變株篩選功能：可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。

?  微藻毒理研究功能：可同時測量96個微藻樣品（對照和處理組）的光合活性，軟件自動給出處理組樣品相對于對照組的光合抑制百分比。

?  吸光系數(shù)測量功能：快速測量葉片的吸光系數(shù)。吸光系數(shù)測量光源： 16個紅光（650 nm）和16個近紅外（780 nm）LED，用于測量植物葉片或藻類樣品PAR吸光系數(shù)。

?  監(jiān)測功能：可實時監(jiān)控箱內(nèi)環(huán)境狀態(tài)、機器狀態(tài)監(jiān)控區(qū)顯示目前程序及環(huán)境狀態(tài)

?  環(huán)境仿真功能：可程序化多組程序功能 (可多至6組)，可個別設定執(zhí)行天數(shù)，可設備單日環(huán)境參數(shù)（間隔可至分鐘），程序化環(huán)境參數(shù)

?  項目管理功能：預約存多次測量的植物影像及參數(shù)，延時（縮時）攝影功能

?  數(shù)據(jù)分析功能：可導出照片文件，可導出葉綠素熒光參數(shù)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等

系統(tǒng)設計

?  可單獨作為葉綠素熒光儀使用，方便帶到野外田間測量和成像；也可配置箱體式結(jié)構，容量300L，能同時滿足植物培養(yǎng)和葉綠素熒光成像的功能。箱體內(nèi)設有栽培區(qū)，可分2層、3層、4層等，調(diào)節(jié)高度進行培養(yǎng)和測量。箱體內(nèi)有特殊消光圖層，避免反光造成的測量干擾。

?  系統(tǒng)配置觸摸式高清顯示屏（24寸），方便操作，支持1670萬色彩和FHD高清畫質(zhì)。

?  系統(tǒng)處理器：I7處理器，16G緩存，512固態(tài)硬盤SSD，滿足快速數(shù)據(jù)處理和存儲要求。

?  供電：室內(nèi)使用220V，50Hz交流電，熒光儀單獨使用時，內(nèi)置可充電鋰電池供電，滿足4小時的野外測量

箱體環(huán)境控制（光照、溫度等）

?  溫度環(huán)控條件：15-40°C ±5°C（關燈）

?  光照系統(tǒng)：采用四合一LED光盤，白光5000k ±500k、紅光660nm±10nm、藍450nm±10nm、紅外光730nm±10nm，光盤面積29×40cm，四種光源可獨立自主控制，光質(zhì)強度可達PPFD ≧700µmols-1m-2@15cm，光照時間控制可程序化設定，設定時間可至1min，可仿真日出至日落光照變化。

?  控制系統(tǒng)：PID微電腦控制，可設定上晝夜循環(huán)程序

?  可選配二氧化碳和濕度控制單元

成像技術參數(shù)

Maxi探頭

?  熒光測量光源： 44個藍色LED，450 nm，測量光強度0.5 μmol m-2 s-1PAR，光化光強度1900 μmol m-2 s-1PAR，飽和脈沖強度4000 μmol m-2 s-1PAR

?  吸光系數(shù)測量光源：16個紅光（660 nm）和16個近紅外（780 nm）LED，用于測量樣品PAR吸光系數(shù)。

?  成像面積：工作距離18.5cm，成像面積 10×13 cm；工作距離22.5cm，成像面積11×15cm。

?  光強異質(zhì)性：測量區(qū)域光強異質(zhì)性小于±7％。

?  測量參數(shù)：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等。

應用領域

?  光合作用研究：可以在相同的條件下同時對大量樣品進行成像

?  植物病理學：病斑部位（包括肉眼不可見時）成像以及病斑擴散的時空動力學

?  植物脅迫生 理學：肉眼不可見脅迫損傷的早期檢測

?  遺傳育種：出苗后大規(guī)?？焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株

?  突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等

?  微藻毒理學：不同毒物濃度多個重復的樣品一次測完，軟件自動計算抑制比率

?  分子生物學：宏觀水平上檢測樣品的綠色熒光蛋白（GFP）熒光

?  其它多種擴展研究

成像參數(shù)

 Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

突變株的快速篩選

MAXI-IMAGING-PAM特別適合對幼苗、愈傷組織、微藻等進行突變株的快速篩選，適合于與光合突變株、抗逆（抗旱、抗鹽、抗病等）突變株、產(chǎn)油/氫突變株等的快速篩選。

國外利用MAXI-IMAGING-PAM篩選突變株的典型客戶如拜耳、BASF、孟山都、先正達等大型跨國農(nóng)業(yè)，以及各大農(nóng)業(yè)育種、植物分子生物學等科研單位，例如澳大利亞植物功能基因組中心（阿德雷德大學）、德國尤里希表型植物表型研究中心（Julich Plant Phenotyping Centre）等等。

國內(nèi)約一半的MAXI-IMAGING-PAM客戶在進行突變株快速篩選工作，主要分布于、中國農(nóng)科院和各大高校。

突變株篩選實例一：國內(nèi)某客戶篩選的擬南芥突變株

突變株篩選實例二：產(chǎn)油突變株的篩選。Ajjawi et al, 2010, Plant Physiol., 152: 529-540.

突變株篩選實例三：光合突變株的篩選。Armbruster et al., 2010, Plant Cell, 22: 3439-3460.

調(diào)制葉綠素熒光成像實例

熒光成像與CO2氣體交換的同步測量

（GFS-3000/IM-MAXI）：MAXI-探頭與GFS-3000聯(lián)用，在10 cm x 13 cm的面積上同步測量氣體交換與熒光成像。

產(chǎn)地：德國WALZ

代表文獻

數(shù)據(jù)來源：光合作用文獻Endnote數(shù)據(jù)庫

原始數(shù)據(jù)來源：Google Scholar

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