Nature Nanotechnology 5, 291 - 296 （2010） 

Published online: 14 March 2010 | doi:10.1038/nnano.2010.23

Subject terms: Nanomaterials | Nanomedicine

Three-dimensional tissue culture based on magnetic cell levitation

以下是國內(nèi)相關(guān)媒體報(bào)導(dǎo)：

借助磁懸浮構(gòu)成三維人工肺   日期：2013-02-08 作者：小云 來源：新民晚報(bào)             

對細(xì)胞培植進(jìn)行操控的能力，開啟了培植結(jié)構(gòu)復(fù)雜組織的新方向。就拿這次的肺細(xì)支氣管組織來說，它由4層復(fù)制而成，分別是血管內(nèi)皮細(xì)胞、平滑肌細(xì)胞、成纖維細(xì)胞和上皮細(xì)胞。研究人員說，用磁性懸浮的方式在肺組織內(nèi)安排這些細(xì)胞，得到的是zui接近于對象本人的支氣管組織。這是先前從來沒有做到過的。

三維細(xì)胞培養(yǎng)的新進(jìn)展[創(chuàng)新技巧]  【字體： 大 中 小 】 www.ebiotrade.com 時(shí)間：2010年04月01日 來源：生物通

萊斯大學(xué)生物工程系的副教授Robert Raphael認(rèn)為：“這種方法的美就在于它利用天然的細(xì)胞間相互作用來驅(qū)動(dòng)三維結(jié)構(gòu)的形成。此方法相當(dāng)簡單，任何對藥物開發(fā)、干細(xì)胞或再生醫(yī)學(xué)感興趣的實(shí)驗(yàn)室都可以此作為三維細(xì)胞培養(yǎng)的起點(diǎn)。“

這種三維組織培養(yǎng)有望應(yīng)用在疾病研究上。M.D. Anderson癌癥中心的Wadih Arap教授表示：“對于癌癥研究，磁場所產(chǎn)生的‘隱性支架’已經(jīng)不再是單純的細(xì)胞培養(yǎng)，而讓它們更像真正的腫瘤。我們下一步將應(yīng)用這種磁性來探索腫瘤成像和治療上的應(yīng)用

細(xì)胞培養(yǎng)，讓細(xì)胞有家的感覺作者：小茜來源：生物通2010-3-19 10:09:03

 德克薩斯大學(xué)Anderson癌癥中心的David  H.Koch表示，在癌癥研究中，3D技術(shù)顯得更為關(guān)鍵，我們下一步的計(jì)劃是將這些3D技術(shù)用于癌癥方面的研究。

3D細(xì)胞培養(yǎng)技術(shù)為細(xì)胞提供一個(gè)更加接近體內(nèi)生存條件的微環(huán)境，感覺就像“在家里一樣”。該技術(shù)不僅僅應(yīng)用于基于細(xì)胞的高通量藥物篩選，還可以應(yīng)用于醫(yī)學(xué)中。

 這個(gè)設(shè)備是目前zui簡單實(shí)用的3D細(xì)胞培養(yǎng)裝置，在6孔或24孔或96孔板中，加入您研究的細(xì)胞核營養(yǎng)介質(zhì)及添加磁納米顆粒，細(xì)胞粘附在磁納米顆粒后，在培養(yǎng)板上面放置磁鐵驅(qū)動(dòng)塊，細(xì)胞在磁性牽引下，在懸浮狀態(tài)下互相靠近，并進(jìn)行重新排列，產(chǎn)生自己的細(xì)胞外基質(zhì)，形成多細(xì)胞球樣體。

以下是部分論文標(biāo)題：

Tissue Engineering C Multitype Bronchiole Model publication reports findings of Dr. Jane Grande-Allen, a professor at Rice University, and Dr. Glauco Souza of n3D on a study that demonstrates the effectiveness of 3D cell culturing by magnetic levitation in creating a multitype bronchiole co-culture model using the Bio-Assembler™.   | 26 Feb 2013 |

Tissue Engineering C publication reports findings of Dr. Mikhail Kolonin, an associate professor at the University of Texas Health Science Center at Houston, and Dr. Glauco Souza of n3D on study that demonstrates how effective 3D cell culturing by magnetic levitation is in generating white adipose tissue organoids or adipospheres, using the Bio-Assembler™.   | 2 Nov 2012 |

  Adipose tissue engineering in three-dimensional levitation tissue culture system based on magnetic nanoparticles

Atherosclerosis publication cites “Detection of Hydroxyapatite in Calcified Cardiovascular Tissues” using n3D’s 3D cell-culturing methods. | 24 July 2012 |羥基磷灰石檢測心血管鈣化組織

n3D’s White Paper provides an overview and technical notes related to key results when “3D Cell Culturing with the Bio-Assembler”. | 01 Apr 2012 |

note: for the complete version of this White Paper, with Technical Notes and referencescontact us.

Nature Nanotechnology publication reports findings on n3D’s method for “Three-dimensional tissue culture based on magnetic levitation”. . | 14 March 2010 |三維細(xì)胞組織培養(yǎng)磁懸浮裝置

Neoplasia publication reports findings from n3D’s collaborators at MD Anderson Cancer Center for “Invasive glioblastoma cells acquire stemness and increased Akt activation”.   Volume 12 | Number 6 | June 2010

小鼠神經(jīng)干細(xì)胞，間充質(zhì)干細(xì)胞，牙髓干細(xì)胞，內(nèi)皮細(xì)胞，平滑肌細(xì)胞，上皮細(xì)胞，成纖維細(xì)胞，軟骨細(xì)胞，與細(xì)胞分離脂肪，血管內(nèi)皮細(xì)胞，骨髓源內(nèi)皮祖細(xì)胞，心肌細(xì)胞，骨骼肌細(xì)胞，  面頜肌細(xì)胞 ，成骨細(xì)胞 ，牙齦成纖維細(xì)胞 ，牙周膜成纖維細(xì)胞，骨髓間充質(zhì)干細(xì)胞，破骨細(xì)胞，軟骨細(xì)胞，血管平滑肌細(xì)胞，血管內(nèi)皮細(xì)胞，動(dòng)脈平滑肌細(xì)胞，靜脈平滑肌細(xì)胞，動(dòng)脈內(nèi)皮細(xì)胞，靜脈內(nèi)皮細(xì)胞，

bone marrow endothelial cells ， murine neural stem cells, mesenchymal stem cells,  dental pulp stem cells，endothelial, smooth muscle, epithelial, fibroblasts, chondrocytes, and cells isolated from adipose