蘇州阿爾法生物實(shí)驗(yàn)器材有限公司
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蘇州阿爾法生物實(shí)驗(yàn)器材有限公司
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所 在 地蘇州市
聯(lián)系方式：耿少華查看聯(lián)系方式
更新時(shí)間：2023-06-20 08:25:06瀏覽次數(shù)：436次
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經(jīng)營(yíng)模式：經(jīng)銷(xiāo)商
商鋪產(chǎn)品：841條
所在地區(qū)：江蘇蘇州市
聯(lián)系人：耿少華 （經(jīng)理）
細(xì)胞名稱(chēng)：人慢性髓原白血*細(xì)胞（STR鑒定正確）k562 細(xì)胞細(xì)胞簡(jiǎn)稱(chēng)：K-562細(xì)胞別稱(chēng)：K562; K.562; K 562; KO; GM05372; GM05372E產(chǎn)品貨號(hào)：TCH-C226規(guī)格：1×10?cells/T25 培養(yǎng)瓶
k562 細(xì)胞
該細(xì)胞適合做轉(zhuǎn)染宿主，來(lái)自于一位53歲女性慢性粒細(xì)胞白血*人的胸腔積液。
細(xì)胞名稱(chēng)：人慢性髓原白血*細(xì)胞（STR鑒定正確） k562 細(xì)胞
細(xì)胞簡(jiǎn)稱(chēng)：K-562
細(xì)胞別稱(chēng)：K562; K.562; K 562; KO; GM05372; GM05372E
產(chǎn)品貨號(hào)：TCH-C226
規(guī)格：1×10?cells/T25 培養(yǎng)瓶
種屬來(lái)源：人
組織來(lái)源：骨髓
特征：慢性骨髓性白血*
細(xì)胞形態(tài)：淋巴母細(xì)胞樣
生長(zhǎng)特性：懸浮生長(zhǎng)
培養(yǎng)體系：IMDM+10%FBS+1%P/S
配套培養(yǎng)基貨號(hào)：TCH-G226
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：24-48 h
培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存
質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性
k562 細(xì)胞使用說(shuō)明
k562 細(xì)胞 參考文獻(xiàn)：
"Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765
Ortaldo JR, et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J. Natl. Cancer Inst. 59: 77-82, 1977. PubMed: 69036
Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. PubMed: 163658
Lozzio BB, Lozzio CB. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk. Res. 3: 363-370, 1979. PubMed: 95026
Andersson LC, et al. K562--a human erythroleukemic cell line. Int. J. Cancer 23: 143-147, 1979. PubMed: 367973
Lozzio BB, et al. A multipotential leukemia cell line (K-562) of human origin. Proc. Soc. Exp. Biol. Med. 166: 546-550, 1981. PubMed: 7194480
Dimery IW, et al. Variation amongst K562 cell cultures. Exp. Hematol. 11: 601-610, 1983. PubMed: 6576909
Chan YJ, et al. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus. J. Virol. 70: 5312-5328, 1996. PubMed: 8764042
Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663
Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842"
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