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聯(lián)系我時,請告知來自 儀表網(wǎng)人宮頸癌細(xì)胞-HeLa細(xì)胞是一個來自人體組織經(jīng)連續(xù)培養(yǎng)獲得的非整倍體上皮樣細(xì)胞系,它由GeyGO等在1951年從31歲女性黑人的宮頸癌組織建立。已知該細(xì)胞系含有人HPV病毒HPV18序列,需在2級生物安全防護(hù)臺操作。該細(xì)胞角蛋白陽性,p53表達(dá)量較低。
人宮頸癌細(xì)胞-HeLa細(xì)胞
人宮頸癌細(xì)胞-HeLa細(xì)胞是一個來自人體組織經(jīng)連續(xù)培養(yǎng)獲得的非整倍體上皮樣細(xì)胞系,它由GeyGO等在1951年從31歲女性黑人的宮頸癌組織建立。已知該細(xì)胞系含有人HPV病毒HPV18序列,需在2級生物安全防護(hù)臺操作。該細(xì)胞角蛋白陽性,p53表達(dá)量較低,但表達(dá)正常水平的pRB(視網(wǎng)膜母細(xì)胞瘤抑制因子)。
細(xì)胞名稱:人宮頸癌細(xì)胞
細(xì)胞簡稱:HeLa 細(xì)胞
細(xì)胞別稱:HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri
產(chǎn)品貨號:TCH-C193
規(guī)格:1×10?cells, T25 培養(yǎng)瓶
種屬來源:人
組織來源:宮頸
特征:宮頸癌
細(xì)胞形態(tài):上皮細(xì)胞樣
生長特性:貼壁生長
培養(yǎng)體系:MEM+10%FBS+1%P/S
人宮頸癌細(xì)胞-HeLa細(xì)胞 配套培養(yǎng)基貨號: TCH-G193
傳代比例:1:2-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲存
質(zhì)量檢測:細(xì)菌、真菌、支原體檢測均為陰性
實(shí)驗(yàn)室儀器:二氧化碳培養(yǎng)箱
實(shí)驗(yàn)耗材:T25細(xì)胞培養(yǎng)瓶,T75培養(yǎng)瓶,錐形瓶
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Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844
Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740
Schneider-Gadicke A, Schwarz E. Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 5: 2285-2292, 1986. PubMed: 3023067
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Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218
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