RhizoCab是一個(gè)由鋁合金和玻璃組成的成像室(W x D x H = 1.5 m x 1.5 m x 2.5 m),內(nèi)部配有465 nm、525 nm和625 nm三組特殊LED光源,側(cè)面固定一臺(tái)高分辨率相機(jī)(蔡司50 mm f/2 Makro-Planar 鏡頭和Basler raL12288-8gm相機(jī)),該相機(jī)可以根據(jù)實(shí)驗(yàn)需求進(jìn)行移動(dòng)。當(dāng)傳送系統(tǒng)將RhizoTube送至位置后,利用自動(dòng)旋轉(zhuǎn)裝置進(jìn)行360°旋轉(zhuǎn),與此同時(shí)相機(jī)會(huì)對(duì)整個(gè)RhizoTube高度范圍內(nèi)進(jìn)行成像,對(duì)于一個(gè)給定的波長(zhǎng),成像時(shí)間大約10秒。此外,您還可以將相機(jī)定焦于某個(gè)感興趣的區(qū)域,以便獲得更高分辨率的圖像。
Jeudy C, Adrian M, Baussard M, Bernard C, Bernaud E, Bourion V,Busset H, Cabrera-Bosquet L, Cointault F, Han S, Lamboeuf M, Moreau D, Pivato B, Prudent M, Trouvelot S, Truong HN, Vernoud V, Voisin AS, Wipf D and Salon C(2017)RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation, Plant Methods, 2016,https://doi.org/10.1186/s13007-016-0131-9