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2'-Deoxy-2'-fluoronucleosides & Derivatives
Synthetic RNA oligonucleotides, incorporating 2'-Deoxy-2’-fluoro-nucleosides (2’-F-RNA) find constantly increasing applications in research and new drug development. Among important areas of their application are such as design and synthesis of efficient and stable siRNAs, aptamers, anti-microRNAs (Antagomirs) as well as general use for affinity purification of RNA-binding proteins. Today Metkinen Chemistry offers more than 99% pure purine 2’-fluoronucleosides, prepared by bioconversion of synthetic 2’-F-uridine, using evolved recombinant E.coli strains. These strains, in turn are grown on the media, incorporation only ingredients, derived from yeast and vegetable. That means that in the production process no secondary reagents of animal source (either BSE certified or non- BSE certified) are employed at any stage of 2’-F- A and G preparation. Hence, we guarantee the complete absence of ANY BSE/TSE risk, associated with our 2’-F-nucleosides and derivatives. In addition to purine 2’-F-nucleosides, we offer a full set of 2’-F-nucleoside CE-phosphoramidites for chemical synthesis and a full set of 2’-F-nucleoside 5’-triphosphates for enzymatic preparation of 2’-F-RNA. Our latest addition to series of 2’-fluoronucleoside derivatives – 2’-F-nucleoside bound solid supports for 2’-F-RNA oligosynthesis, complements the range of these compounds and opens up a full spectrum of 2’-F-RNA applications. 2'-Fluoro modifications consistently provided increased target binding affinity, endonuclease stability, and, importantly, reduced immune response. In addition, it is the only modification compared to other carbohydrate modifications, including locked nucleic acid, or LNA, that preserves RISC activity when used in both sense and antisense strands. 2’-Deoxy-2’-fluoroguanosine 2’-Deoxy-2’-fluoroadenosine 2’-Deoxy-2’-fluoroguanosine-3’-CE-phosphoramidite 2’-Deoxy-2’-fluoroadenosine-3’-CE-phosphoramidite 2’-Deoxy-2’-fluorouridine-3’-CE-phosphoramidite 2’-Deoxy-2’-fluorocytidine-3’-CE-phosphoramidite
2’-Deoxy-2’-fluoroarabinonucleosides (FANA) & Derivatives
2’-Fluoroarabinooligonucleotides (FANA) can be used as antisense oligonucleotides, siRNA and aptamers. Today Metkinen Chemistry offers synthetically synthesized pyrimidine 2’-fluoroarabinonucleosides as well as purine 2’- fluoroarabinonucleosides, prepared by bioconversion of synthetic 2’-F-arabinouridine, employing propriarory evolved recombinant E.coli strains. We guarantee the complete absence of ANY BSE/TSE risk, associated with our 2’-F- fluoroarabinonucleosides. Metkinen Chemistry offers a full set of 2’-F-nucleoside CE-phosphoramidites for the chemical synthesis and a full set of 2’-F-nucleoside 5’-triphosphates for enzymatic preparation of FANA. 9-(2-Deoxy-2-fluoro-ß-D-arabinofuranosyl)adenine (2’-FANA-A) 9-(2-Deoxy-2-fluoro-ß-D-arabinofuranosyl)-2,6-diaminopurine (FANA-DAP) 9-(2-Deoxy-2-fluoro-ß-D-arabinofuranosyl)guanine (2’-FANA-G) 9-(2-Deoxy-2-fluoro-ß-D-arabinofuranosyl)hypoxantine (2’-FANA-I) 1-(2-Deoxy-2-fluoro-ß-D-arabinofuranosyl)cytosine (2’-FANA-C) 1-(2-Deoxy-2-fluoro-ß-D-arabinofuranosyl)uracil (2’-FANA-U) 9-(2-Deoxy-2-fluoro-ß-D-arabinofuranosyl)-6-thioguanine (FANA-thioG) 2'-Deoxy-2'-fluoroarabinoadenosine-3'-CE-phosphoramidite 2'-Deoxy-2'-fluoroarabinoguanosine-3'-CE-phosphoramidite 2'-Deoxy-2'-fluoroarabinouridine-3'-CE-phosphoramidite 2'-Deoxy-2'-fluoroarabinocytidine-3'-CE-phosphoramidite

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