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The LightSwitch Promoter Reporter GoClone Collection

Our comprehensive collection contains more than 18,000 endogenous human promoter reporter GoClones that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity.

PRODUCTSDESCRIPTIONCONTROL VECTORSPRODUCT SPECSRESOURCES

SwitchGear offers a number of control constructs to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that SwitchGear’s unique RenSP luciferase technology eliminates the need for co-transfection of a normalizing control in most cases.

Ordering

Promoters of housekeeping genes (Positive controls) Details
Product #	Name	Description	Coordinates	Ordering
S708908	RPL10_PROM	PROM CONTROL	Get info	5ug ($230) 10ug ($450)
S717678	ACTB_PROM	PROM CONTROL	Get info	5ug ($230) 10ug ($450)
S721624	GAPDH_PROM	PROM CONTROL	Get info	5ug ($230) 10ug ($450)
S721613	LDHA_PROM	PROM CONTROL	Get info	5ug ($230) 10ug ($450)
Random genomic fragments in promoter vector (Negative/Background controls) Details
Product #	Name	Description	Coordinates	Ordering
S790001	R01_PROM	RAND CONTROL	Get info	5ug ($230) 10ug ($450)
S790002	R02_PROM	RAND CONTROL	Get info	5ug ($230) 10ug ($450)
S790003	R03_PROM	RAND CONTROL	Get info	5ug ($230) 10ug ($450)
S790004	R04_PROM	RAND CONTROL	Get info	5ug ($230) 10ug ($450)
Empty promoter vector
Product #	Name	Description	Coordinates	Ordering
S790005	EMPTY_PROM	EMPTY VECTOR	Get info	FREE*	5ug

* One free empty vector construct (5ug) with any purchase

If you are interested in ordering more than 10ug of one or more control constructs, please contact our Sales Department for a custom quote.

Details

Empty promoter vector: The empty promoter vector contains the RenSP luciferase gene without a promoter. This construct serves as a one measure of background signal in the experiment. This construct may also be used to create additional experimental constructs.

Housekeeping gene promoter vectors: Housekeeping control constructs contain promoters for common housekeeping genes driving the RenSP luciferase reporter. These constructs serve as positive transfection controls and may also serve as controls for comparing signals between conditions if they are known to be unresponsive to the test condition.

Random control vectors: Random control constructs contain 1 kb non-conserved, non-genic, and non-repetitive fragments from the human genome cloned upstream of the luciferase reporter. These vectors produce slightly different signals than the empty vectors and are considered optimal negative or background controls.

	Figure 1. Expected data and analysis of housekeeping and random promoter vectors: Random promoter constructs (R1, R2, R3, R4) serve as negative/background controls and establish baseline luciferase activity. The housekeeping promoter controls serve as positive controls and give relative luciferase activity.
Y-axis values shown in log scale. Control vector activity may vary depending on cell line, treatment condition, and protocol changes.

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