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Anti-RUBISCO抗體免費(fèi)代測(cè)

時(shí)間:2014-4-2閱讀:382
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   Anti-RUBISCO抗體免費(fèi)代測(cè)

FROM: ABCAM

Catalog Number : BYK-6988R       
Quantity size :   100ug  (0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide)

Anti-RUBISCO抗體免費(fèi)代測(cè)Background: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.

Sequence similarities: Belongs to the RuBisCO large chain family. Type I subfamily.
Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.

Specificity :   

  • Anti-RUBISCO is a rabbit polyclonal antibody unconjugated                
  • specific for RUBISCO of Arabidopsis Thaliana          
  • use for western blotting,elisa,immunoprecipitation and immunohistochemistry
  • Protein G affinity chromatography purification, purity :>95%
  • Isotype:  IgG
  • molwt:  52 kDa

Anti-RUBISCO抗體免費(fèi)代測(cè)Application :  

  • Western blotting 1:100-500                                
  • ELISA 1:500-1000
  • IHC-P=1:100-500
  • IHC-F=1:100-500 
  • IF= 1:50-200                  
  • Optimal working dilutions must  be determined by the end user.

Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4

Anti-RUBISCO抗體免費(fèi)代測(cè)Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

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