背景介紹：Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins. 

Function:
E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane. 
P7 forms a leader sequence to properly orient NS2 in the membrane. 
Uncleaved NS2-3 is required for production of infectious virus. 
NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. 
NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. 
NS4A is a cofactor for the NS3 protease activity. 
RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.

別名：CSFV Polyprotein; CSFV E2 protein; CSFV E2; polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; CSFV Envelope glycoprotein E2; Classical swine fever virus E2; Classical swine fever virus Envelope glycoprotein E2; structural protein E2

豬瘟病毒E2蛋白抗體,Anti-CSFV E2抗體簡(jiǎn)單介紹：

產(chǎn)品編號(hào)：BYK-10765R

英文名稱：Anti-CSFV E2
中文名稱：豬瘟病毒E2蛋白抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來(lái)源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請(qǐng)或添加客服直接索取）

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

豬瘟病毒E2蛋白抗體,Anti-CSFV E2抗體產(chǎn)品信息：

免疫組化問(wèn)題解答

染色過(guò)強(qiáng)？

原因：抗體濃度過(guò)高或孵育時(shí)間過(guò)長(zhǎng)

解決方法：降低抗體滴度、抗體孵育時(shí)間：室溫1小時(shí)或4度過(guò)夜

原因：孵育溫度過(guò)高超過(guò)37度:

解決方法：一般在室溫20-28度

原因：DAB顯色時(shí)間過(guò)長(zhǎng)或濃度過(guò)高?

解決方法：顯色時(shí)間不超過(guò)5-12分鐘，以顯微鏡下觀察為準(zhǔn)

非特異性背景染色？

原因：操作過(guò)程中沖洗不充分

解決方法：每步?jīng)_洗3次每次5分鐘

原因：組織中含過(guò)氧化物酶未阻斷 

解決方法：可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng) 

原因：組織中含內(nèi)原性* 

解決方法：正常非免疫動(dòng)物血清再封閉

原因：血清蛋白封閉不充分 

解決方法：血清蛋白封閉不充分

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