背景介紹：In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

別名：Ac-lysine; acetyl Lysine; Lysine.

乙?；嚢彼峥贵w，Anti-acetyl Lysine抗體簡單介紹：

產(chǎn)品編號(hào)：BYK-8850R

英文名稱：Anti-acetyl Lysine
中文名稱：乙?；嚢彼峥贵w

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請(qǐng)或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

乙酰基賴氨酸抗體，Anti-acetyl Lysine抗體產(chǎn)品信息：

博研生物小課堂：

常用激光器對(duì)應(yīng)熒光素的選擇：

藍(lán)色激光器熒光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

紅色激光器熒光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黃色激光器熒光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

免疫組化結(jié)果的判斷

對(duì)免疫組化結(jié)果的判斷應(yīng)持科學(xué)的慎重態(tài)度，要準(zhǔn)確判斷陽性和陰性，排除假陽性和假陰性結(jié)果，必須嚴(yán)格對(duì)照實(shí)驗(yàn)，對(duì)新發(fā)現(xiàn)的陽性結(jié)果，除有對(duì)照實(shí)驗(yàn)結(jié)果之外，應(yīng)進(jìn)行多次重復(fù)實(shí)驗(yàn)，可用幾種方法進(jìn)行驗(yàn)證。必須學(xué)會(huì)判斷特異性染色和非特異性染色，對(duì)初學(xué)者更為重要，否則會(huì)得出不科學(xué)的結(jié)論。特異性染色和非特異性染色的鑒別點(diǎn)主要在于特異性反應(yīng)產(chǎn)物常分布于特定的部位。如胞漿內(nèi)，也有分布在細(xì)胞核和細(xì)胞表面的，即具有結(jié)構(gòu)性。特異性染色表現(xiàn)為在同一切片上呈現(xiàn)不同程度的陽性染色結(jié)果。非特異性染色表現(xiàn)為無一定的分布規(guī)律，常呈某一部位成片的均勻著色，細(xì)胞和周圍的結(jié)締組織均無區(qū)別的著色，或結(jié)締組織呈現(xiàn)很強(qiáng)的染色。非特異性染色常出現(xiàn)在干燥切片的邊緣，有刀痕或者折疊的部位。在過大的組織塊，中心固定不良也會(huì)導(dǎo)致非特異性染色。有時(shí)可見非特異性染色和特異性染色同時(shí)存在，由于過強(qiáng)的非特異性染色背景不但影響對(duì)特異性染色結(jié)果的觀察和記錄，而且令人對(duì)其特異性結(jié)果產(chǎn)生懷疑。

免疫組化的呈色深淺可反映抗原存在的數(shù)量，可作為定性、定位和定量的依據(jù)。

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