背景介紹：Host cell factor (HCF) is a cellular cofactor required for the activation of VP16 which expresses the herpes simplex virus immediate early gene. V16 binds to HCF through a 4-amino acid motif similar to the HCF binding domain of the basic leucine-zipper proteins Luman and Zhangfei (ZF). Luman activates promoters containing cAMP or unfolded protein response elements (UPRE). Zhangfei suppresses the transcriptional activity of Luman, but requires HCF binding which may target Luman and Zhangfei to a common location. Sequence analysis predicts that the deduced 272-amino acid Zhangfei protein has a negatively charged N terminus, a leucine zipper of 6 heptad leucine repeats separated by a conserved 6-amino acid spacer, a basic domain, and a bZIP region. It is also presumed that the N-terminal acidic region of Zhangfei is an activation domain.

別名：1110034C16Rik; 6330417B10Rik; AI225749; AI606244; CREB/ATF bZIP transcription factor; Crebzf; FLJ94018; HCF-binding transcription factor Zhangfei; Host cell factor-binding transcription factor Zhangfei; LAZip; LAZipII; SHP-interacting leucine zipper protein; SMILE; ZF; ZHANG_HUMAN. 

宿主細(xì)胞因子Zhangfei抗體，Zhangfei抗體簡(jiǎn)單介紹：

產(chǎn)品編號(hào)：BYK-19162R

英文名稱：Anti-Zhangfei
中文名稱：宿主細(xì)胞因子Zhangfei抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來(lái)源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請(qǐng)或添加客服直接索取）

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

宿主細(xì)胞因子Zhangfei抗體，Zhangfei抗體產(chǎn)品信息：

博研生物小課堂：

常用激光器對(duì)應(yīng)熒光素的選擇：

藍(lán)色激光器熒光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

紅色激光器熒光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黃色激光器熒光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

1.陽(yáng)性細(xì)胞染色分布有三種類型：細(xì)胞漿、細(xì)胞核、細(xì)胞膜表面。大部分抗原見于細(xì)胞漿，可見于整個(gè)胞漿或部分胞漿

2.陽(yáng)性細(xì)胞分布可分為灶型和彌漫性

3.由于細(xì)胞內(nèi)含抗原量不同，所以染色強(qiáng)度不一。如果細(xì)胞之間染色強(qiáng)度相同，常提示其反應(yīng)為非特異性

4.陽(yáng)性細(xì)胞染色定位于單個(gè)細(xì)胞，且與陰性細(xì)胞相互交雜分布；而非特異性染色常不限于單個(gè)細(xì)胞，而是累及一片細(xì)胞。

5.切片邊緣、刀痕或褶皺區(qū)域，壞死或擠壓的細(xì)胞區(qū)，膠原結(jié)締組織等，常表現(xiàn)為相同的陽(yáng)性染色強(qiáng)度，不能用于判斷陽(yáng)性。

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