【文獻標(biāo)題】Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress 
【作者】J. LIN, J.P. LI, F. YUAN,et.al
【作者單位】山東師范大學(xué)（Shandong Normal University）
【文獻中引用產(chǎn)品】
植物鎂螯合酶(MgCh) ELISA試劑盒
植物Rubisco活化酶(RCA)ELISA試劑盒
【關(guān)鍵詞】biomass; greening; ion concentrations; photosynthetic parameters; plant height. 
【DOI】doi.org/10.1007/s11099-018-0824-6
【影響因子(IF)】2.3
【出版期刊】《PHOTOSYNTHETICA》
【產(chǎn)品原文引用】
Protein content and activity of magnesium chelatase and Rubisco:
Magnesium chelatase and Rubisco were prepared by first freezing a 0.2-g leaf sample in liquid nitrogen to prevent proteolytic activity, followed by grinding with 2 mL of extraction buffer (100 mM phosphate buffer, pH 7.2, containing 0.1 mM EDTA, and 2 mM ascorbic acid). The homogenate was centrifuged for 15 min at 10,000 rpm, and the supernatant was used to measure the protein content (ng L–1), magnesium chelatase activity [pmol(Mg-Deutero) min–1 mg–1(protein)] and Rubisco activity [μmol min–1 mg–1(protein)]. Magnesium chelatase and Rubisco were measured using a magnesium chelatase content kit and a Rubisco content kit (double antibody sandwich method, Huding Biological Technology Co., Shanghai, China) (3 replicates per treatment). 

完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員！
更多公司福利請關(guān)注“上海滬鼎”VX公眾號！