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Anti-ERK1抗體技術(shù)支持

時(shí)間:2014/11/27閱讀:454
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Anti-ERK1抗體

 

Catalog Number : BYK-1020R      
Quantity size :   0.1ml 
(1mg/ml,Lyophilized or Liquid.Buffer=0.01M PBS,ph 7.4 with 10 mg/ml BSA and 0.1% Sodium azide.Reconstitute with 0.1ml sterile distilled water.)

 

Anti-ERK1抗體background: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4(HSF4) (By similarity). Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung. Increased expression during development. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

 

Specificity :   

  • Anti-ERK1 is a rabbit Polyclonal antibody unconjugated                 
  • specific for ERK1 of Human, Mouse, Rat,
  • use for western blotting,elisa,immunoprecipitation and immunohistochemistry
  • Protein G affinity chromatography purification, purity :>95%
  • Isotype:  IgG
  • mol wt:  43kDa

 

Anti-ERK1抗體Application :  

  • Western blotting=1:100-500                 
  • Immunohistocry 1:100-500                
  • ELISA= 1:500-1000  
  • IHC-P=1:100-500  IHC-F=1:100-500 
  • Optimal working dilutions must be determined by the end user.

 

Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4

 

Anti-ERK1抗體Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

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