Anti-CD34/FITC抗體產(chǎn)品咨詢

Catalog Number : BYK-2038R-FITC      
Quantity size :   100ug (2mg/1ml, Lyophilized or Liquid. Buffer = 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide. Reconstitute with 50ul sterile distilled water.)

Background:  The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

Also known as: CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN; Hematopoietic Stem Cell Marker; Endothelial Markers; Tumor biomarkers.

Specificity:   -Rabbit Polyclonal IgG, affinity purified by Protein A.

-FITC conjugated

-Reacts with: Human, Mouse, Rat 

-Immunogen: KLH conjugated synthetic peptide derived from human CD34.

-Predicted molecular weight: 39kDa

Application:  Flow-Cyt=1:50-200  IF=1:50-200

            Excitation spectrum: 490-495nm

            Emission spectrum: 520-530nm

            Not yet tested in other applications.

            Optimal working dilutions must be determined by the end user.

Storage:     Store at -20 °C for one year. Avoid repeated freeze/thaw

            cycles. The Lyophilized or Liquid antibody is stable at room

            temperature for at least one month and for greater than a year when

            kept at -20 °C. When reconstituted in sterile pH 7.4 0.01M PBS or

            diluent of antibody the antibody is stable for at least two weeks at

            2-4 °C.

Important Note: This product as supplied is intended for research

             use only, not for use in human, therapeutic or diagnostic

             applications..