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Anti-Cyclin A2抗體說明書

時間:2014-11-18閱讀:529
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Anti-Cyclin A2抗體說明書

Catalog Number : BYK-0571R       
Quantity size :   0.1ml 
(1mg/ml,Lyophilized or Liquid.Buffer=0.01M PBS,ph 7.4 with 10 mg/ml BSA and 0.1% Sodium azide.Reconstitute with 0.1ml sterile distilled water.)

 

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

 

Specificity :   

  • Anti-Cyclin A2 is a rabbit polyclonal antibody unconjugated                
  • specific for Cyclin A2 of Human, Mouse, Rat  
  • use for western blotting,elisa,immunoprecipitation and immunohistochemistry
  • Protein G affinity chromatography purification, purity :>95%
  • Isotype:  IgG
  • mol wt:  49kDa

 

Application :  

  • Western blotting=1:100-500                
  • Immunohistochemistry 1:100-500                
  • ELISA= 1:500-1000  
  • IHC-P=1:100-500  IHC-F=1:100-500 
  • Optimal working dilutions must be determined by the end user.

 

Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4

 

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

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