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產(chǎn)品名稱：豬雌二醇（E2）ELISA試劑盒實(shí)實(shí)在在    
英文名稱：PoFAine Estradiol,E2  ElisaKit.
產(chǎn)品編號(hào)：BYS12624B
產(chǎn)品性狀：液體
產(chǎn)品用途：科研實(shí)驗(yàn)
產(chǎn)品規(guī)格：96T/48T

ELISA實(shí)驗(yàn)原理: 用純化的抗體包被微孔板，制成固相載體，往包被抗體的微孔中依次加入標(biāo)本或者標(biāo)準(zhǔn)品、*化的抗體、HRP-標(biāo)記的親和素，經(jīng)過*洗滌后用底物顯色。底物在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色， 并在酸的作用下轉(zhuǎn)化成zui終的黃色。 顏色的深淺和樣品中的目標(biāo)蛋白呈正相關(guān)。用酶標(biāo)儀在（450nm）波長(zhǎng)下測(cè)定測(cè)定O.D.值（吸光度），計(jì)算樣品濃度。.

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The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase（HRP） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve