Anti-SRC抗體,src原癌基因抗體說(shuō)明書(shū)產(chǎn)品詳細(xì)資料

產(chǎn)品編號(hào) BYK-1135R

英文名稱(chēng) SRC
中文名稱(chēng) src原癌基因抗體
別    名 ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specific protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; Protooncogene SRC; protooncogene SRC Rous sarcoma; Src; SRC Oncogene; SRC_MOUSE; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC 1; Tyrosine protein kinase SRC1; v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog; V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian); v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian; vsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog.
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號(hào)  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  激酶和磷酸酶  表觀(guān)遺傳學(xué)  
分 子 量 61kDa

抗體來(lái)源 Mouse or Rabbit
克隆類(lèi)型 Monoclonal or Polyclonal
產(chǎn)品應(yīng)用 WB、ELISA、IHC-P、IHC-F、Flow-Cyt、IF、IP、ICC 此產(chǎn)品應(yīng)用不做依據(jù)，具體產(chǎn)品應(yīng)用與實(shí)驗(yàn)稀釋比請(qǐng)！

not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
亞    型 IgG
純化方法 affinity purified by Protein G
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.


     Anti-SRC抗體,src原癌基因抗體說(shuō)明書(shū)產(chǎn)品介紹 

      c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.  

 此相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記 

     相關(guān)產(chǎn)品：

     Anti-GNAI2抗體,G蛋白α抑制蛋白1抗體

Anti-GNRPX抗體,*核苷酸結(jié)合蛋白X抗體
Anti-GPKOW抗體,G蛋白修補(bǔ)結(jié)構(gòu)域蛋白5抗體
Anti-GRAMD2抗體,GRAM結(jié)構(gòu)域蛋白2抗體
Anti-GRAMD3抗體,丙型肝炎病毒-NS3反轉(zhuǎn)錄激活蛋白2抗體
Anti-GSTCD抗體,*硫轉(zhuǎn)移酶C段結(jié)構(gòu)域抗體
Anti-GTDC1抗體,糖基轉(zhuǎn)移酶樣1抗體
Anti-GTPBP9抗體,*核苷酸結(jié)合蛋白9抗體
Anti-GLT8D1抗體,糖基轉(zhuǎn)移酶8結(jié)構(gòu)域1抗體
Anti-GLTPD2抗體,糖脂轉(zhuǎn)移蛋白結(jié)構(gòu)域2抗體
Anti-Beta galactosidase抗體,β半乳糖苷酶抗體
Anti-Gibberellins抗體,赤霉素抗體
Anti-Gibberellins抗體,?；撬?β-氨基乙磺酸抗體
Anti-GFAP delta抗體,膠質(zhì)纖維酸性蛋白GFAPδ抗體
Anti-Gigaxonin抗體,巨軸索神經(jīng)病蛋白GAN抗體
Anti-Gliomedin抗體,神經(jīng)膠質(zhì)蛋白(肝癌相關(guān)基因2)抗體
Anti-GJC2抗體,間隙連接蛋白47抗體
Anti-GIPC-2抗體,胞漿區(qū)相關(guān)蛋白GIPC2抗體
Anti-VP3 Goose parvovirus抗體,鵝細(xì)小病毒GPV抗體
Anti-PCDHGA1抗體,原鈣粘蛋白γA1抗體