Product Name：Cellscript T7標準RNA體外轉(zhuǎn)錄試劑盒

INTRODUCTION：

The T7-Scribe™ Standard RNA IVT Kit is specially formulated to enable users to obtain the maximum  possible yields of canonical (GAUC) RNA from an in vitro transcription (IVT) reaction. The standard  2 hour, 20 μl reaction will yield up to 150 μg of RNA from 1 μg of the control template. These yields are  made possible by the high-performance properties of the T7-Scribe enzyme. The T7-Scribe Standard RNA IVT Kit produces exceptionally high yields of either long or short transcripts.  The standard reaction can be scaled up to produce milligram amounts of RNA. In addition, a T7-Scribe  reaction can be readily modified to prepare fluorescent-, biotinylated- or digoxigenin-labeled RNA. T7-Scribe IVT RNA can be processed into mRNA (5'-end capped and 3'-end poly(A) tailed) through the  use of CELLSCRIPT's ScriptCap™ m7 G Capping System, ScriptCap 2'-O-Methyltransferase Kit and  A-Plus™ Poly(A) Polymerase Tailing Kit (available separately).  CELLSCRIPT also offers the INCOGNITO™ line of transcription kits for the production of pseudouridine- & 5-methyl-cytosine-containing (GA C and GA 5mC) IVT RNA. It has been shown that -mRNAs and  5mC-mRNAs are translated into protein at higher levels and induce lower innate immune responses in  human and other mammalian cells that express various RNA sensors compared to corresponding  unmodified mRNAs.