MOUSE Anti-AKT/PKB

FORM:ABCAM
Catalog Number : BYK-0115R       
Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent )

 Background: IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. [subcellular location] Cytoplasmic and membrane-associated after cell stimulation leading to its translocation. In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. Belongs to the Ser/Thr protein kinase family. RAC subfamily.

Specificity :   

• Anti-AKTis a mouse monoclonal antibody unconjugated                
• specific for AKT of  Human,
• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt: 56kDa

 Application :  

• Western blotting 1:100-500                
• Immunohistochemistry 1:100-500                 
• ELISA 1:500-1000
•  KLH conjugated synthetic peptide derived from human PKB C-terminus                  
• Optimal working dilutions must  be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

 Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.