Anti-AGER/FITC抗體產(chǎn)品咨詢

Catalog Number : BYK-4999R-FITC      
Quantity size :   100ug (2mg/1ml, Lyophilized or Liquid. Buffer = 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide. Reconstitute with 50ul sterile distilled water.)

Background:  Advanced glycosylation end product-specific receptor (AGER; RAGE) is a member of the immunoglobulin superfamily of cell surface molecules that binds molecules that have been irreversibly modified by non-enzymatic glycation and oxidation, and are know as advanced glycation end products (AGEs). It is expressed by endothelium, mononuclear phagocytes, neurons and smooth muscle cells. Whereas RAGE is present at high levels during development, especially in the central nervous system, its levels decline during maturity.The increased expression of RAGE is associated with several pathological states, such as diabetic vasculopathy, neuropathy, retinopathy and other disorders, including Alzheimer's disease and immune/inflammatory reactions of the vessel walls. In diabetic tissues, the production of RAGE is due to the overproduction of AGEs that eventually overwhelm the protective properties of RAGE. This results in oxidative stress and endothelial cell dysfunction that leads to vascular disease in diabetics. In the brain, RAGE also binds amyloid beta (Ab). Because Ab is overproduced in neurons and vessels in the brains of Alzheimer disease, this leads to the hyperstimulation of RAGE. The RAGE-Ab interaction is thought to result in oxidative stress leading to neuronal degeneration.

Also known as: Advanced glycosylation end product specific receptor; Advanced glycosylation end product-specific receptor; AGER; EC 2.7.11.22; LE 9211 A antigen;LE-9211-A antigen; MGC22357; MOK; RAGE 1; RAGE1; MOK protein kinase; Receptor for advanced glycation endproducts;Renal tumor antigen 1; Renal tumor antigen; Renal cell carcinoma antigen (MOK protein kinase); Renal tumor antigen 1.

Specificity:   -Rabbit Polyclonal IgG, affinity purified by Protein A.

-FITC conjugated

-Reacts with: Human, Mouse, Rat, Dog, Pig, Cow

-Immunogen: KLH conjugated synthetic peptide derived from human AGER.

-Predicted molecular weight: 42kDa

Application:   Flow-Cyt=1:50-200 ICC=1:50-200

IF=1:50-200

              Excitation spectrum: 490-495nm

              Emission spectrum: 520-530nm

              Not yet tested in other applications.

              Optimal working dilutions must be determined by the end user.

Storage:      Store at -20 °C for one year. Avoid repeated freeze/thaw

             cycles. The Lyophilized or Liquid antibody is stable at room

             temperature for at least one month and for greater than a year when

             kept at -20 °C. When reconstituted in sterile pH 7.4 0.01M PBS or

             diluent of antibody the antibody is stable for at least two weeks at

             2-4 °C.

Important Note: This product as supplied is intended for research

              use only, not for use in human, therapeutic or diagnostic

              applications..