Anti-CDK2抗體產(chǎn)品咨詢

Catalog Number : BYK-0757R       
Quantity size :   0.1ml  (1mg/ml,Lyophilized or Liquid.Buffer=0.01M PBS,ph 7.4 with 10 mg/ml BSA and 0.1% Sodium azide.Reconstitute with 0.1ml sterile distilled water.)

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Specificity :   

• Anti- CDK2 is a rabbit polyclonal antibody unconjugated                
• specific for CDK2 of Human, Mouse, Rat  
• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:  33kDa

Application :  

• Western blotting=1:100-500                
• Immunohistochemistry 1:100-500                
• ELISA= 1:500-1000  
• IHC-P=1:100-500  IHC-F=1:100-500 
• Optimal working dilutions must be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.