3t3-l1 細(xì)胞

細(xì)胞名稱：3t3-l1 細(xì)胞Ppp2r1b基因敲除株

細(xì)胞簡(jiǎn)稱：3T3-L1 KO-Ppp2r1b

產(chǎn)品貨號(hào)：CGKO-M2175

參考價(jià)格：15187元

修飾基因：Ppp2r1b Gene (ID: 73699)

修飾類型：敲除

轉(zhuǎn)導(dǎo)方法：慢病毒轉(zhuǎn)導(dǎo)

抗性基因：Hygro

克隆類型：純合子

細(xì)胞鑒定：PCR，測(cè)序

種屬來源：小鼠

組織來源：胚胎

細(xì)胞形態(tài)：成纖維細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

3t3-l1 細(xì)胞培養(yǎng)體系：DMEM-H+10%CS（新生牛血清）+1%P/S

傳代比例：1:3-1:4，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

  3t3-l1 細(xì)胞 是從3T3細(xì)胞（Swissalbino）中經(jīng)克隆分離得到的連續(xù)傳代的亞系。該細(xì)胞從快速分裂到匯合和接觸性抑制狀態(tài)經(jīng)歷了前脂肪細(xì)胞到脂肪樣細(xì)胞的轉(zhuǎn)變。該細(xì)胞鼠痘病毒陰性；可產(chǎn)生甘油三酯，高濃度血清可增強(qiáng)細(xì)胞內(nèi)脂肪堆積.

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