Hut78細(xì)胞

細(xì)胞名稱： 人T淋巴細(xì)胞白血病細(xì)胞（STR鑒定正確）

細(xì)胞簡稱：Hut78細(xì)胞

細(xì)胞別名： Hut 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78;

HUT78; NCI-H78

貨號：TCH-C218

規(guī)格：1×10?cells/ T25 培養(yǎng)瓶

價(jià)格：1400元 (干冰運(yùn)輸需另加200元干冰費(fèi))  

種屬來源：人

組織來源：皮膚

特征：塞扎里綜合癥

細(xì)胞形態(tài)：淋巴母細(xì)胞

生長特性：懸浮生長

培養(yǎng)體系：IMDM+20%FBS+1%P/S

細(xì)胞配套培養(yǎng)基 貨號：TCH-G218

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性

NCI-H78 細(xì)胞 可產(chǎn)生IL-2，TNF-α；表達(dá)IL-2受體

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O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285

Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082

Hut 78 was derived from peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2, and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78.

Cancer Cell 31:225-239(2017)