mcf7細(xì)胞

細(xì)胞名稱(chēng)： 人乳腺癌細(xì)胞（STR鑒定正確）

細(xì)胞簡(jiǎn)稱(chēng)： MCF-7細(xì)胞

細(xì)胞別名： MCF 7; MCF.7; MCF-7；MCF7; Michigan Cancer Foundation-7; ssMCF-7;  ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL；

mcf7細(xì)胞貨號(hào)：TCH-C247

規(guī)格：1×10?cells/T25 培養(yǎng)瓶

參考價(jià)格：1500元  (干冰運(yùn)輸需另加200元干冰費(fèi)) 

種屬來(lái)源：人

組織來(lái)源：乳腺

特征：乳腺癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系： MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+10 µg/mL Human recombinant insulin+1%P/S

mcf7細(xì)胞 培養(yǎng)基 貨號(hào)：TCH-G247

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

mcf7細(xì)胞 的生長(zhǎng)受到腫瘤壞死因子α（TNF alpha）的抑制?？梢酝ㄟ^(guò)用抗雌激素治療來(lái)調(diào)節(jié)IGFBP的分泌。

參考文獻(xiàn)：

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

Takahashi K, Suzuki K. Association of insulin-like growth-factor-I-induced DNA synthesis with phosphorylation and nuclear exclusion of p53 in human breast cancer MCF-7 cells. Int. J. Cancer 55: 453-458, 1993. PubMed: 8375929

Brandes LJ, Hermonat MW. Receptor status and subsequent sensitivity of subclones of MCF-7 human breast cancer cells surviving exposure to diethylstilbestrol. Cancer Res. 43: 2831-2835, 1983. PubMed: 6850594

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Pratt SE, Pollak MN. Estrogen and antiestrogen modulation of MCF7 human breast cancer cell proliferation is associated with specific alterations in accumulation of insulin-like growth factor-binding proteins in conditioned media. Cancer Res. 53: 5193-5198, 1993. PubMed: 7693333

Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088