hela 細(xì)胞

細(xì)胞名稱(chēng)： hela 細(xì)胞 （STR鑒定正確）

細(xì)胞別稱(chēng)：HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri

貨號(hào)：TCH-C193

規(guī)格： 1X10^6

細(xì)胞簡(jiǎn)稱(chēng)：HeLa

種屬來(lái)源：人

組織來(lái)源：宮頸

疾病特征：宮頸癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：MEM+10%FBS+1%P/S

配套培養(yǎng)基貨號(hào)：TCH-G193

傳代比例：1:2-1:6，每2-3天換液一次

hela 細(xì)胞傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

        hela 細(xì)胞是一個(gè)來(lái)自人體組織經(jīng)連續(xù)培養(yǎng)獲得的非整倍體上皮樣細(xì)胞系，它由GeyGO等在1951年從31歲女性黑人的宮頸癌組織建立。已知該細(xì)胞系含有人乳頭狀瘤病毒HPV18序列，需在2級(jí)生物安全防護(hù)臺(tái)操作。該細(xì)胞角蛋白陽(yáng)性，p53表達(dá)量較低，但表達(dá)正常水平的pRB（視網(wǎng)膜母細(xì)胞瘤抑制因子）。

參考文獻(xiàn)：

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