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NUCLEODUR® C18 Pyramid

High purity silica phase for HPLC

Octadecyl modification with polar endcapping,
suitable for aqueous eluents · endcapped · 14 % C · USP L1

Properties

產(chǎn)品應(yīng)用:

鰻魚中的“硝基呋喃"的檢測
色譜柱：NUCLEODUR C18 Pyramid 150×2.0mm 3um流動相：乙腈：0.1% 乙酸溶液（梯度洗脫）流速：0.3毫升/分鐘溫度：35℃ 樣品含量：0.5微克/公斤檢測器：MS

LC-MS/MS 分離尿液中β2—agonists
色譜柱：㏄ 70×4mm NUCLEODUR C18 Pyramid,5um樣品制備：please refer to Thevisetal.,J.Mass Spectrom(2003),38:1197—1206流動相：（A）：5m M乙酸銨，0.1% 乙酸調(diào)節(jié)PH3.5 （B）：乙腈
梯度洗脫：0 99% B in 6 min,在平衡到99% A for 3.5 min流速：0.8毫升/分鐘進樣量：20 ul
溫度：25℃檢測器：ABI2000樣品：1.Reproterol 2.Fenoterol 3.Ritodrine 4.Ractopamine 5.Clenbuterol 6.Bambuterol 7.Mapenterol

核酸色譜柱：EC 250×4mm NUCLEODUR C18 Pyramid,5 um流動相：A)50m M NH4H2PO4,PH2.5 B)乙腈
梯度洗脫A/B 100/0—2.5 min—A/B 100/0—10 min A/B 90/10流速：1.0毫升/分鐘
進樣量：3 ul溫度：25℃檢測器：UV 254nm 樣品：1.胞嘧啶 2.腺嘌呤 3.脲嘧啶 4.雜質(zhì) 5.胸腺嘧啶

尿素，雙縮脲和三聚氰胺的分析

色譜柱：㏄ 250×4mm NUCLEODUR C18 Pyramid,5um

樣品制備：Urea(c=0.5mg/ml),Biuret(c=0.09mg/ml),Cyanuric Acid(c=0.05mg/ml)

流動相：純水（99%）

流速：1.0毫升/分鐘

進樣量：10 ul

檢測器：UV 190nm

樣品：1.Urea 尿素 2.Biuret雙縮脲 3.不純物 4.Cyanuric Acid (c=0.05mg/ml)三聚氰胺

作者：Greve.C.

來源：Institutfur Chemische Verfahrenstechnik,Universitat Clausthal,2005

訂貨信息:

EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water

Length ->	30 mm		50 mm		75 mm		100 mm		125 mm		150 mm		250 mm
NUCLEODUR® C18 Pyramid, 1.8 μm
2 mm ID	760271.20		760272.20		760275.20		760273.20				760274.20
3 mm ID	760271.30		760272.30				760273.30
4 mm ID	760271.40		760272.40				760273.40
4.6 mm ID	760271.46		760272.46				760273.46
EC guard columns*:			4 x 2 mm		761915.20				4 x 3 mm		761915.30
NUCLEODUR® C18 Pyramid, 3 μm
2 mm ID			760263.20				760264.20		760260.20		760261.20		760262.20
3 mm ID			760263.30				760264.30		760260.30		760261.30		760262.30
4 mm ID			760263.40				760264.40		760260.40		760261.40		760262.40
4.6 mm ID			760263.46		760259.46		760264.46		760260.46		760261.46		760262.46
EC guard columns*:			4 x 2 mm		761916.20				4 x 3 mm		761916.30
CC guard columns**:			8 x 3 mm		761854.30				8 x 4 mm		761854.40
NUCLEODUR® C18 Pyramid, 5 μm
2 mm ID			760200.20				760204.20		760201.20		760203.20		760202.20
3 mm ID			760200.30				760204.30		760201.30		760203.30		760202.30
4 mm ID			760200.40				760204.40		760201.40		760203.40		760202.40
4.6 mm ID			760200.46		760205.46		760204.46		760201.46		760203.46		760202.46
EC guard columns*:			4 x 2 mm		761917.20				4 x 3 mm		761917.30
CC guard columns**:			8 x 3 mm		761800.30				8 x 4 mm		761800.40
Guard columns in packs of 3, EC columns in packs of 1
Guard columns for EC columns						EC columns with ID:								Use guard column holder
						2 mm		3 mm		4 mm		4.6 mm
*		Column Protection System		EC guard column		4/2		4/3		4/3		4/3		REF 718966
**		ChromCart® guard column system		CC guard column		8/3		8/3		8/4		8/4		REF 721359
Custom-packed EC columns with other dimensions are available on request.
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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water

Length	10 mm ID	21 mm ID	32 mm ID	40 mm ID
NUCLEODUR® C18 Pyramid, 5 μm
50 mm	762271.100	762271.210
125 mm	762273.100	762273.210
150 mm				762269.400
250 mm	762272.100	762272.210	762272.320	762272.400
VP guard columns with NUCLEODUR® C18 Pyramid
	8 mm ID	16 mm ID	32 mm ID
10 mm*	762291.80	762291.160
15 mm*			762293.320

* VP guard column	Size	Pack of	for VP column	required holder	REF
VP 10/8	10 x 8 mm ID	2	10 mm ID	8 mm	718251
VP 10/16	10 x 16 mm ID	2	21 mm ID	16 mm	718256
VP 15/32	15 x 32 mm ID	1	32–40 mm ID	32 mm	718253
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.

Standard program* of columns packed with NUCLEODUR® C18 Pyramid


Column system		Particle size:	1.8 μm	3 μm	5 μm
Analytical	EC columns (standard system, 2–4.6 mm)
Preparative	VarioPrep columns (VP, ≥ 8 mm)
if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.